This LabCoP Diagnostic Network Optimisation (DNO) Sub-CoP ECHO session in co-convened with the Foundation for Innovative New Diagnostics (FIND), focuses on providing an overview of the DNO analysis in Zambia for TB/HIV testing integration, including objectives, methodology and key findings. It further explores how findings from the diagnostic network optimization analysis informed Zambia Ministry of Health’s strategies to improve access to TB and HIV testing for vulnerable population groups, and looked at perspectives for uptake and sustainability for proposed diagnostic network improvements. Presentations were made by Powel Choonga, Laboratory Advisor, Ministry of Health, Zambia; and Trevor Machila, Monitoring & Evaluation Manager, Centre for Infectious Disease Research in Zambia (CIDRZ). Please follow the links here to view the recorded ECHO session and download the presentation slides.