On 7 June 2022, this special ECHO session was held to focus on the Monkeypox virus outbreak. In this session, led by Dr Jacqueline Weyer, Principal Medical Scientist, Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, National Health Laboratory Service, South Africa; and Dr Placide Mbala, Head of the Epidemiology Department and Pathogen Genomic Laboratory, National Institute for Biomedical Research (INRB), Democratic Republic of the Congo discussed what we know and do not yet know about the monkeypox outbreak and its implications for the public health and laboratory response in Africa. This session also featured current developments on the multinational outbreak of monkeypox, clinical features, laboratory diagnosis, management of cases, and public health measures to contain the spread of monkeypox. Please follow the links here to view the recorded ECHO session and download the presentation slides.