The Stop TB/USAID/GLI Practical Guide to Implementation of Truenat Tests has been updated to include best practices and lessons learned from countries that have implemented Truenat under the introducing New Tools Project (annex 13). This version also includes a new checklist for a Truenat testing site supervisory visit (annex 12). The Practical Guide to Implementation of Truenat Tests provides practical guidance to plan for the adoption and implementation of the WHO-recommended Truenat tests for the rapid detection of TB and rifampicin resistance. It includes advice on how to translate findings from the WHO policy guidance into an actionable implementation plan, combined with operational considerations on use of the technology and initial field experience from early implementers. The guide also compiles as annexes a number of resources that can be easily adopted by programmes and sites, including a sample SOP for running the assays, checklists for planning and sites assessments, and job aids. The guide was developed in collaboration with the Stop TB Partnership and USAID.