Tuberculosis (TB) remains a major public health challenge. The World Health Organization (WHO) recommends three classes of nucleic acid amplification tests (NAATs) for initial TB detection: near point-of-care, low-complexity, and medium-complexity platforms. While this expanded toolkit improves access to rapid diagnosis, it also adds complexity. Implementing a diversified diagnostic network requires data-driven planning and careful matching of tools to population needs. Close collaboration between National TB Programs (NTPs) and National TB Reference Laboratories (NTRLs) is essential to ensure that diagnostic strategies are aligned with network capacity and priorities. Decisions must balance cost, coverage, and equitable access to services. A comprehensive, costed implementation plan should guide all stages of deployment, from infrastructure and workforce capacity to training, data systems, and monitoring. This guide distils key insights and practical lessons from South Africa, Kenya, and Uganda, where multiple molecular diagnostic platforms have been successfully implemented to strengthen TB detection and expand access to timely diagnosis.