In this Antimicrobial (AMR) Community of Practice (CoP) webinar on 11 October 2024, we focus on the European Committee on Antimicrobial Susceptibility Testing (EUCAST), tracing its evolution, organization, and its critical role in standardizing antimicrobial susceptibility testing (AST) globally. It delves into EUCAST’s methodology for setting clinical breakpoints, the tools and guidance available for phenotypic testing, and the importance of integrating public consultation and scientific consensus into decision-making. The presentation also contrasts EUCAST’s approach with other global bodies, emphasizing its impact on public health through standardized testing, effective surveillance of antimicrobial resistance (AMR), and the collaboration between human and veterinary health sectors. Attendees will gain valuable insights into the process of developing breakpoints, the significance of tools like WHONET, and the role of EUCAST in shaping global antimicrobial resistance policies.