Mozambique provided this Excel-based tool, with its accompanying instructions, as a country example of a comprehensive diagnostic network optimisation or mapping tool at the July 2019 Global Diagnostics Integration meeting in Geneva. The government sought a tool that could be: subnational, consider multiple potential technologies as well as both laboratory-based and point-of-care, incorporate testing across several diseases (HIV, tuberculosis (TB), hepatitis B and C, and HPV), ensure both patient and operational considerations, consider both current and future demand, and is easy to use. Product and site selection is a critical step to ensuring sustainable and optimal diagnostic integration. These are often completed or supported through country-driven, patient-focused, comprehensive, and transparent diagnostic network optimization or mapping exercises. Several tools, some of which are open access, currently exist to support diagnostic network optimization.